Relative bioavailability of organic and hydroxy copper sources in growing steers fed a high antagonist diet1
Abstract
To assess relative bioavailability (RBV) of hydroxy and organic Cu sources compared with CuSO4 in steers fed a high Cu antagonist diet, 84 steers were stratified by BW to pens randomly assigned to dietary treatments for 90 d. Steers received a common corn silage-based diet supplemented with Cu antagonists (diet analyzed 0.25% S; 6.8 mg Mo/kg DM). Supplemental (SUPP) Cu treatments included: control (CON; no SUPP Cu), low or high inorganic (ING5 or ING10; 5 or 10 mg Cu/kg DM from CuSO4; Old Bridge Chemical Inc., Old Bridge, NJ, USA), low or high organic (ORG5 or ORG10; 5 or 10 mg Cu/kg DM from Cu lysine; CuPlex 100, ZinPro Corp., Eden Prairie, MN), and low or high hydroxy (HYD5 or HYD10; 5 or 10 mg Cu/kg DM; IntelliBond CII, Micronutrients USA LLC, Indianapolis, IN). Body weights were recorded on days -7, -6, 28, 56, 84, and 85, and plasma samples collected on days -7, 28, 56, and 85. Liver samples were collected to start and end the trial. Data were analyzed using the mixed procedure of SAS and the model included treatment with initial liver Cu values used as a covariate in analysis of final liver Cu. Contrast statements were used to separate treatment means: 0 vs. 5 mg SUPP Cu/kg DM, 0 vs. 10 mg SUPP Cu/kg DM, 5 vs. 10 mg SUPP Cu/kg DM, HYD vs. ORG, HYD vs. ING, and ORG vs. ING. Initial liver Cu concentrations were similar across all treatment comparisons (P ≥ 0.22). Final liver Cu concentrations were lesser in CON compared with either 5 or 10 mg Cu/kg DM (P ≤ 0.001). Final…
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Evidence Hub reads this as a randomized trial. Its evidence level is 2 of 5 (High · randomized trial). Randomized trials of a reasonable size are the strongest single-study design.
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