Analysis of Metabolites by GC-MS and FTIR Spectroscopy Techniques to Ethanolic Whole Plant Extract from Thymus Vulgaris and Assessment of its Antibacterial Potency
Abstract
Background: The use of medicinal plants has been crucial in the treatment of a wide range of illnesses. The medicinal herb thymus vulgaris has a long history of biological and pharmacological applications. Small chemicals called metabolites are involved in metabolic activities that are critical for the growth, maintenance, and function of cells. Metabolite concentrations typically span multiple orders of magnitude, and their molecular weights are typically 50–1500 Da. Metabolites are quite sensitive to a wide range of environmental factors, and the metabolome is itself very dynamic and time-dependent. Objectives: Our research aims were: Ethanolic whole-plant extract of Thymus vulgaris was analysed phytochemically and identified using Fourier Transform Infrared Spectroscopy (FTIR). Utilising the Gas Chromatography-Mass Spectrometry (GC-MS) Method for Fate Profile Analysis. Assessing Its Antimicrobial Effects. Materials and Methods: The dried plant parts, Thymus vulgaris, were sourced from the local markets of Babylon Province. After cleaning and isolating foreign substances, they were studied at the advanced Botanical laboratory at the College of Science, University of Babylon. Following their crushing by an electrical grinder, the powder was gathered in nylon bags and stored at room temperature in the laboratory until needed. A GC-MS was used for separation and identification and KBr to experimentally prepare for FTIR analysis. All experimental samples were run through three…
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