Use of secondary enrichment for isolation of Salmonella from naturally contaminated environmental samples
Abstract
Since the implementation of Hazard Analysis Critical Control Point (HACCP), the need for on-farm food safety risk assessment and management has greatly increased. In order to provide accurate risk assessments, attention should be focused on better characterization of the Salmonella isolation and identification techniques. In this work, we compared the isolation ability of 4 Salmonella-specific protocols: immunomagnetic separation (DB), tetrathionate (TT) broth, Rappaport-Vassiliadis R10 (RV) broth, and a secondary enrichment (TR) procedure as well as 2 selective solid media (brilliant green agar, BG; and xylose-lysine tergitol 4, XLT4). All 4 methods were compared in litter and drag swab samples that were collected weekly during the broiler grow out period in 7 houses. There were 65/126 (51.6%) pooled litter samples positive and 115/304 (37.8%) drag swab samples positive for Salmonella by at least one method. Of the 65 positive litter samples, DB, RV, and TT isolated 1 (2.7%), 31 (47.7%), and 23 (35.4%) of the samples as positive when using BG agar, respectively. The TR protocol identified 83.1% (54/65) of the positive samples as positive when using BG agar. In the drag swab samples, DB did not identify any samples as positive, whereas TT and RV found 28 (25.7%) and 26 (23.9%) of the 109 samples to be positive when using BG agar, respectively. Again, the TR protocol identified the highest percentage of positive samples (94.5%). An analysis of agreement, kappa, revealed that…
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