Simultaneous determination of dexamethasone and moxifloxacin in pharmaceutical formulations using stability indicating HPLC method
Abstract
Abstract A simple, sensitive, specific, precise and accurate stability indicating reverse phase liquid chromatographic method was developed for simultaneous determination of moxifloxacin hydrochloride and dexamethasone in bulk drugs and pharmaceutical formulations. The developed chromatographic method was optimized for the separations of moxifloxacin hydrochloride, dexamethasone and stress-induced degradation products by the use of BDS Hypersil C8 column (250 × 4.6 mm, 5 μm) as stationary phase with mobile phase consisting of a mixture of phosphate buffer (20 mM) containing 0.1% (v/v) triethylamine, at pH 2.8 (adjusted with dilute phosphoric acid) and methanol (38.5:61.5 v/v) at a flow rate of 1.5 mL min−1. Chromatographic separations of analytical peaks and degradation products were achieved within 10 min. Detection of the analytes and degradation products was performed at 254 nm using a diode array detector. Method validation was performed in accordance with ICH guidelines. Linearity of the method was established over the concentration ranges of 50–350 μg mL−1 for moxifloxacin hydrochloride and 10–70 μg mL−1 for dexamethasone (correlation coefficients greater than 0.999). The method resulted in good separation of both the analytes and degradation products with acceptable tailing and resolution with peak purity index ⩾0.9999 which indicated complete separation of analyte peaks from the degradation products. The method can therefore be considered as stability-indicating and…
Read the paper
Related studies
- Synthesis, In Vitro and In Silico Molecular Docking Studies of Novel Phthalimide–Pyrimidine Hybrid Analogues to Thalidomide as Potent Antitubercular Agents
- Exploring the inhibitory performance of expired moxifloxacin and norfloxacin on copper corrosion in saline environment
- Molecular investigation of antimicrobial peptides against Helicobacter pylori proteins using a peptide-protein docking approach
- Screening of novel narrow-spectrum benzofuroxan derivatives for the treatment of multidrug-resistant tuberculosis through in silico, in vitro, and in vivo approaches
- Synthesis, Characterization, Anticancer, Antioxidant, and In Silico Evaluation of Semicarbazides and Thiosemicarbazides
- Fast and accurate prediction of drug induced proarrhythmic risk with sex specific cardiac emulators
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.