Synergistic moxifloxacin detection: AgNPs/GO electrochemical immunosensor with oriented antibody immobilization
Abstract
Moxifloxacin (MXF), a broad-spectrum fluoroquinolone antibiotic, is widely used in human and veterinary medicine. However, its overuse leads to environmental residues and antimicrobial resistance, posing significant health risks. Herein, a label-free electrochemical immunosensor was developed for ultrasensitive MXF detection using silver nanoparticle-graphene oxide nanocomposites (AgNPs/GO). The synergistic effects of GO (high conductivity) and AgNPs (redox activity) enhanced electron transfer efficiency. Critically, Staphylococcal protein A (SPA) enabled oriented Fc-directed antibody immobilization, significantly enhancing antigen-binding efficiency and sensitivity compared to random adsorption. Material characterization (UV-Vis, XRD, SEM, TEM) confirmed the nanostructure. The sensor exhibited a wide linear range (0.50-200 ng/mL), ultralow detection limit (0.18 ng/mL, S/N = 3), and high specificity against structural analogs. Practical validation in spiked pork and milk samples achieved recoveries of 95-104 % with long-term stability (>82.5 % signal retention over 28 days). This work provides a reliable platform for monitoring antibiotic residues in food safety systems.
Read the paper
Related studies
- Synthesis, In Vitro and In Silico Molecular Docking Studies of Novel Phthalimide–Pyrimidine Hybrid Analogues to Thalidomide as Potent Antitubercular Agents
- Exploring the inhibitory performance of expired moxifloxacin and norfloxacin on copper corrosion in saline environment
- Molecular investigation of antimicrobial peptides against Helicobacter pylori proteins using a peptide-protein docking approach
- Screening of novel narrow-spectrum benzofuroxan derivatives for the treatment of multidrug-resistant tuberculosis through in silico, in vitro, and in vivo approaches
- Synthesis, Characterization, Anticancer, Antioxidant, and In Silico Evaluation of Semicarbazides and Thiosemicarbazides
- Fast and accurate prediction of drug induced proarrhythmic risk with sex specific cardiac emulators
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.