Drug susceptibility of Mycobacterium avium isolated from patients with mycobacteriosis with positive and negative HIV status
Abstract
Objective.
Comparative assessment of in vitro susceptibility of M. avium isolated from patients with mycobacteriosis (MB) with positive and negative HIV status.
Materials and Methods.
M. avium isolates of were revealed during 2018–2023 from clinical samples of 402 adult patients (≥ 18 years old) in the National Medical Research Center for Phthisiopulmonology and Infectious Diseases of the Ministry of Health of the Russian Federation: 144 strains were isolated from individuals with HIV infection (HIV+) and 258 – from patients with a negative HIV status (HIV-). Susceptibility was determined for 15 antimicrobials: amikacin, clarithromycin, rifabutin, rifampin, ethambutol, streptomycin, ciprofloxacin, doxycycline, isoniazid, ethionamide, linezolid, moxifloxacin, trimethoprim-sulfamethoxazole, bedaquiline, reviewed using the broth microdilution method in accordance with the CLSI M24-A2, 2018 guidelines. Antimicrobial susceptibility categories were determined based on minimum inhibitory concentration (MIC) breakpoints according to CLSI M24S, 2023 standards. For amikacin, clarithromycin, linezolid, and moxifloxacin, M. avium complex breakpoints were used. For ciprofloxacin, doxycycline, rifabutin, and rifampin, MIC breakpoints for other slow-growing NTMs (M. kansasii and M. marinum) according to CLSI M24S, 2023 Non-Species Breakpoints were used. For ethambutol, isoniazid, streptomycin, ethionamide, bedaquiline, delamanid, MIC breakpoints for slowgrowing NTM have not been…
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