Soil-Dwelling Bacteria Display Tolerance Against Typical Antibiotic Serum Levels in an Ex Vivo Model of Traumatized Tissue
Abstract
Introduction: Military wounds often contain large amounts of necrotic, avascular tissue that provide an ideal home for environmental and endogenous flora to dwell. Debridement mitigates infection but cannot be performed until the casualty is transported to a surgically equipped site, sometimes days after initial injury. Meanwhile, opportunistic pathogens spread throughout the wound site. Systemic antibiotic therapies fail to eradicate these soil microorganisms potentially because they dwell in an antibiotic-tolerant phenotype. Laboratory tests that incorporate environment soil may promote the development of point-of-care therapies effective against biofilm-like organisms. We hypothesized that, in an ex vivo model of traumatized tissue, blood-serum antibiotic concentrations would fail to eradicate soil-dwelling organisms.
Materials and methods: Sterile, excised tissue was collected from concurrent sheep studies. Tissue samples were coated with topsoil that was confirmed to contain naturally occurring viable organisms, then blasted with an air cannon to simulate trauma and distribute soil throughout. The tissue test environment was then moved into a 6 well-plate for treatment with saline suspensions of systemic levels of antimicrobials, or a 10× concentration of the same. Following a 24-hour incubation, tissues and remaining treatment was diluted and quantified on selective and non-selective agar plates. Colony forming units (CFU)/g of tissue were calculated between agar type…
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