Analgesic Efficacy of Oral Carprofen-Treated Gel and Tablets for an Incisional Pain Model in NSG Mice
Abstract
Stress in research animals affects their welfare and alters physiologic responses, potentially impacting research outcomes. Laboratory rodents often experience stress from repeated parenteral drug administrations due to handling and needle punctures. In this study, we compared the efficacy of oral carprofen tablets and carprofen-treated nutritional gels compared with injectable carprofen for alleviation of postoperative mechanical and thermal hypersensitivity in NSG mice. Male and female mice (n = 59) were randomly assigned to one of 5 groups: 1) placebo (Placebo tablets or NutraGel, daily); 2) carprofen tablets (CarpTab; 2 mg, daily); and 3) carprofen injection (Carp25SC; 25 mg/kg SC, daily), low-dose carprofen-treated gels (CarpGel-low; 0.11 mg/mL, daily); and high-dose carprofen-treated gels (CarpGel-high; 0.22 mg/mL, daily). Mechanical and thermal hypersensitivities were assessed before surgery on D-1 and at D0 (4 h), D1, and D2 afterward. Plasma carprofen concentration (n = 56) was assessed over 1 to 4 d. Daily clinical observations, fecal occult blood testing, and gross necropsies were performed. Results showed that mechanical and thermal hypersensitivity persisted in the placebo group throughout the study (D0 to D2). CarpTab and Carp25SC groups effectively attenuated mechanical hypersensitivity compared with the baseline (D0 to D2). Compared with baseline, CarpGel-low attenuated mechanical hypersensitivity on D1 and CarpGel-high attenuated mechanical hypersensitivity…
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