Analysis of the gut microbiome of HIV-infected patients using 16s rRNA sequencing
Abstract
Background: the microbiota of the gastrointestinal tract is one of the important factors that ensure the normal functioning of the human body. Patients with HIV infection are most susceptible to intestinal infections and changes in the composition of the microbiota, both due to exposure to the virus itself and as a result of reduced immunity. This article describes changes in the composition of the intestinal microflora of patients with HIV infection at the stage of secondary diseases, receiving antiretroviral therapy and not.
Aims: to determine the composition of the gut microbiome in patients with HIV infection on and off antiretroviral therapy using DNA sequencing of the hypervariable regions of the 16S rRNA gene.
Materials and methods: the object of the study was a group of patients in the amount of 10 people with a documented confirmed diagnosis of HIV infection and diarrheal syndrome. In order to clarify a possible etiological factor and determine the characteristics of the microbiocenosis of the gastrointestinal tract, stool analysis was performed using the 16s rRNA sequencing method.
Results: the study revealed significant changes in the composition of the microbiome: depletion of the Bacteroidetes type, increased number of the Proteobacteria type. A statically significant difference was found in the composition of the types of microbiota in healthy people and patients with HIV infection (p0.05). The normal flora was represented by only two microorganisms:…
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