Abstract B14: Flow cytometry for targeted culturomics of gut commensal species and rapid overview of microbiota composition
Abstract
An ever-increasing number of studies report the importance of the composition of the intestinal microbiota in the treatment of cancers. The mechanisms involved are varied. The reciprocal interaction of drugs with bacteria can have an effect on the efficacy as well as the toxicity of treatments. In addition, some species or strains are able to stimulate CD8+ T cell production and the effectiveness of immune checkpoint blockers (ICBs). As part of these treatments, there is therefore a potential interest in being able to isolate and cultivate bacterial species associated with the response to ICBs in different cancers. The objective is to better understand the mechanisms involved and possibly develop complementary therapeutic and/or diagnostic solutions. However, this approach is still limited by the fact that a large number of commensal bacterial species in the intestine are difficult to cultivate, due to specific nutritive requirements, extreme oxygen sensitivity (EOS), or under-representation in the whole bacterial population. In an attempt to circumvent these limitations, we developed flow cytometry (FCM) and cell sorting in anaerobic conditions. A series of polyclonal antibodies specific to commensal species known to be beneficial to the host such as Faecalibacterium prausnitzii, Akkermansia muciniphila, Christensenella minuta, and Eubacterium hallii have been produced and used in combination with viability and Gram staining to rapidly detect, sort, and culture species of…
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