Ophiopogonin A alleviates acute lung inflammation via NF-κB/MAPK signaling pathway in a mouse model of Klebsiella pneumoniae
Abstract
Klebsiella pneumoniae (Kp) is a gram-negative bacterium present in the flora of the mouth, skin and intestines. Due to the emergence of antibiotic resistance, the outcome of Kp pneumonia is becoming worse. Exploring an effective treatment method is clinically important. Ophiopogon
japonicus is a traditional Chinese herbal medicine for treating inflammation and oxidative stress damage, as ophiopogonin A (OP-A) is its main active ingredient. We investigated the impact of OP-A on Kp pneumonia and further elucidated the underlying mechanism. After the establishment
of Kp mouse model, the animals were grouped and received intraperitoneal injection of OP-A, levofloxacin (Lvx) or PBS. After 12 hours, mice were dissected to detect the pathological changes of lung tissues, and the number of inflammatory proteins and inflammatory cells in the bronchoalveolar
lavage fluid (BALF). Finally, Western blot and RT-qPCR analyses were carried out to detect MARK, JNK, ERK and NF-κB expression in mouse lung tissues upon treatments. Administration of OP-A attenuated the pathological damage of lung tissues of Kp pneumonia mice, as both Lvx and
OP-A significantly controlled and inhibited the wet-to-dry ratio of the lung tissues. Increased protein content and inflammatory cells infiltration were visible in Kp pneumonia mice, while the advent of Lvx and OP-A dramatically diminished inflammatory cells infiltration in BALF of lung tissues
with fewer contents of IL-1β, IL-6 and TNF-α. The protective…
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