Targeted in vitro and in silico assessment of Moringa oleifera leaf extract: inhibitory effects on adipogenesis and enhancement of insulin sensitivity
Abstract
UNLABELLED: This study aimed to explore the medicinal properties of Moringa, a traditional food of the Asian continent, namely India, Bangladesh, the Philippines, and Indonesia. Based on the effectiveness of moringa extract on metabolic disorders, a novel strategy utilizing indicators of the incretin pathway was investigated. Moringa leaf extract (MLE) at 1 mg/ml concentration demonstrated 85% of radical scavenging activity of DPPH radicals and efficient reduction of F2 + in Ferric Reducing Antioxidant Power assay. MLE was authenticated by HPTLC fingerprinting, and UPLC-MS revealed the potent bioactive list. MLE showed anti-adipogenesis property by reducing the lipid accumulation in the adipocytes in comparison with the standard at 250 μg/ml concentration, which was shown in Oil Red O staining. Anti-adipogenesis property was studied by gene expression analysis of PPARγ, SREBP, and C/EBPα genes. The MLE further showed 60% of lipase inhibition at a 1 mg/ml concentration compared to the control orlistat. MLE action on intestinal neuroendocrine cell line demonstrated 60% of DPP4 inhibition and was justified by suppression of DPP4, AMPK, and NQO1 genes by RT-PCR analysis. Further on L-cell, the MLE stimulated the secretion of GLP-1 by 25% at its efficient concentration. The docking studies of potent ligands against DPP4 and lipase enzymes validated the experimental results. These findings indicate that MLE has inhibitory effects on adipogenesis and insulin sensitivity, making it…
Read the paper
Related studies
- Understanding the factors influencing fall armyworm (Spodoptera frugiperda J.E. Smith) damage in African smallholder maize fields and quantifying its impact on yield. A case study in Eastern Zimbabwe
- Alarming situation of emerging H5 and H7 avian influenza and effective control strategies
- Livestock and food security: vulnerability to population growth and climate change
- Atmosphere–ocean oxygen and productivity dynamics during early animal radiations
- Characterization of the global distribution and diversified plasmid reservoirs of the colistin resistance gene mcr-9
- The global seroprevalence of Toxoplasma gondii in pigs: A systematic review and meta-analysis
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.