Novel HPTLC densitometric methods for determination of tamsulosin HCl and tadalafil in their newly formulated dosage form; Comparative study and green profile assessment
Abstract
A promising combination of tamsulosin HCl and tadalafil has been recently introduced for treating two prevalent and associated urological disorders; benign prostate hyperplasia and erectile dysfunction. Novel HPTLC methods were designed and validated for assaying of the cited drugs in their challenging combined formulation. Separation was achieved using HPTLC silica gel 60 F254 plates as a stationary phase with a densitometric measurement at 280 nm. The proposed methods with two different chromatographic systems were successfully applied; a conventional mixture (method I) of ethyl acetate: toluene: methanol: ammonia [5:3:2:0.5, by volume] and a greener one (method II) with ethyl acetate: ethanol: ammonia [8:2:0.1, by volume]. The two methods were evaluated through a comparative study in terms of selectivity, tailing factor, developing time and concentration ranges. The greenness profile for each method was then appraised with several green guides namely; GlaxoSmithKline solvent sustainability guide, environmental, health and safety (EHS) tool, national environmental methods index (NEMI) and Eco-scale. Moreover, method specificity and peak homogeneity were evaluated by peak purity assessment using the winCATS® software spectral correlation tool. The methods have many potentials of being simple, fast, economic, selective and the greener one could be a good option for sustainable analysis of the drugs.
Read the paper
Related studies
- Protective effects of tadalafil and N-acetyl cysteine therapy on cisplatin-induced testicular toxicity
- The Use of Generative AI for Scientific Literature Searches for Systematic Reviews: ChatGPT and Microsoft Bing AI Performance Evaluation
- Sildenafil as a Candidate Drug for Alzheimer’s Disease: Real-World Patient Data Observation and Mechanistic Observations from Patient-Induced Pluripotent Stem Cell-Derived Neurons
- Ozone-modified properties of pumpkin seed oil as anti-H. pylori, anticancer, anti-diabetic and anti-obesity agent
- Effectiveness of Low-Intensity Extracorporeal Shock Wave Therapy in Erectile Dysfunction: An Analysis of Sexual Function and Penile Hardness at Erection: An Umbrella Review
- Molecular Docking of Silymarin as a Ligand for Aromatase Enzyme and HIV-1 Reverse Transcriptase Using Molegro Virtual Docker
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.