Kfc determination after ischemia-reperfusion in lungs treated with sildenafil and tadalafil
Abstract
Ischemia reperfusion phenomenon (IR) is the major factor implicated in primary graft failure, condition that occurs around the 20% of the lung transplants. Some drugs in theory can diminish the IR injury. Sildenafil and tadalafil are drugs that inhibit the 5-Phosphodiesterase, enzyme that inhibits cGMP, leading to vasoconstriction, edema and finally organ failure. The inhibition of 5-phosphodiesterase results in dilatation of pulmonary vasculature and lower hydrostatic pressure, likely decreasing edema formation. The objective of this study was to determinate the edema formation in lungs treated with Sildenafil and tadalafil in animal (ex vivo) model of ischemia-reperfusion. Lung blocks were harvested (Wistar rats) and distributed in the following groups (G): G I: immediately reperfusion. G II: 8 hours preservation, and reperfusion. G III and G IV: Sildenafil (0.7 mg/kg) or tadalafil (0.15 mg/kg) donor pretreatment respectively, 8 hours preservation, and reperfusion. Pulmonary arterial pressure (PAP), pulmonary venous pressure (PVP), lung difference weight (W), pulmonary capillary pressure (PCP) and the capillary filtration coefficient (Kfc) were measured. Results: (mean ± SE). PAP (mmHg): G I: 20.31±1.23, G II: 25.53 ±3.31, G III: 20.78±0.90, G IV: 18.94±1.87. Kfc (ml/min/cmH2O/100gr): G I: 0.18±0.047 G II: 0.99±0.89 G III: 0.34±0.12 G IV: 1.81±0.90*. p=<0.05. Conclusion: Donor pretreatment with sildenafil decrease PAP and edema formation compared with lungs without…
Read the paper
Related studies
- Protective effects of tadalafil and N-acetyl cysteine therapy on cisplatin-induced testicular toxicity
- The Use of Generative AI for Scientific Literature Searches for Systematic Reviews: ChatGPT and Microsoft Bing AI Performance Evaluation
- Sildenafil as a Candidate Drug for Alzheimer’s Disease: Real-World Patient Data Observation and Mechanistic Observations from Patient-Induced Pluripotent Stem Cell-Derived Neurons
- Ozone-modified properties of pumpkin seed oil as anti-H. pylori, anticancer, anti-diabetic and anti-obesity agent
- Effectiveness of Low-Intensity Extracorporeal Shock Wave Therapy in Erectile Dysfunction: An Analysis of Sexual Function and Penile Hardness at Erection: An Umbrella Review
- Molecular Docking of Silymarin as a Ligand for Aromatase Enzyme and HIV-1 Reverse Transcriptase Using Molegro Virtual Docker
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.