39 Effect of Tadalafil on Reduction of Necrosis in the Ischemic Zone in a Rat Comb Burn Model
Abstract
A major goal of burn management is to reduce the progression of necrosis in the zone of ischemia surrounding the central zone of necrosis. A rat comb burn model is used to assess the progression of necrosis in the zone of ischemia. We compared various combinations of naproxen [NPX], N-acetyl cysteine [NAC], and tadalafil [TD] (a phosphodiesterase-6 inhibitor used as a vasodilator to treat erectile dysfunction) in a rat comb burn model to determine their effects on injury progression. We created 2 comb burns on the backs of 40 anesthetized Sprague-Dawley rats using a brass comb with 4 rectangular prongs preheated in boiling water and applied for 30 seconds, resulting in four rectangular 10 × 20mm full-thickness burns separated by three 5 × 20mm unburned interspaces, representing the ischemic zones. We randomized 5 animals each to daily oral gavage with TD (1mg/kg), NPX (10 mg/kg), NAC (500 mg/kg), NAC+NPX, TD+NPX, TD+NAC, TD+NPX+NAC, or normal saline [NS]. Wounds were observed daily for gross evidence of necrosis in the unburned interspaces and full-thickness biopsies from the interspaces were evaluated with H&E 7 days after injury for histological evidence of necrosis. Repeated measures ANOVA demonstrated reduced gross %age of interspace area undergoing necrosis in all groups that included TD, compared with all groups not including TD (P<0.001). There were no differences among the various treatments within the groups that did or did not include TD (Figure). The percentages…
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