Effect of Activated Platelet-Rich Plasma on In Vitro Spermatogenesis in Testicular Organoids
Abstract
Infertility affects 10–20% of couples, and male-related factors contribute to 30–50% of cases. Although testicular organoids (TOs) support in vitro spermatogenesis, one of the most significant limitations of these approaches is the undefined nature of serum and/or xenogenic growth factors used in the culture media. Platelet-rich plasma (PRP) has a regenerative effect on spermatogenesis after testicular damage and maintains the proliferation and function of spermatogonial stem cells (SSCs) in vitro. In this study, prepubertal C57BL/6 testicular cells were cultured with activated PRP using ultra-low attachment and air–liquid interface culture systems. Spermatogenic progression was evaluated at weeks 1, 3, 4, and 6 through histomorphometric and immunohistochemical analyses. In TOs supported with 10% PRP (PRP group), the SSC pool was preserved for 6 weeks, and the number of ID4(+) SSCs was higher than that in TOs supported with 10% knockout serum replacement (KK group) and 5% knockout serum replacement + 5% fetal bovine serum (KF group) at weeks 3, 4, and 6. In the PRP group, the number of SCP3(+) spermatocytes remained constant from weeks 1 to 6 and was higher than that in the KK and KF groups at weeks 4 and 6. In all TOs, ACR(+) round spermatids were observed at week 3, and PRP group had more ACR(+) spermatids than KK group at week 4. The results suggest that activated PRP may serve as a promising culture supplement to support spermatogenesis, although its safety and clinical…
Read the paper
Related studies
- Protective effects of tadalafil and N-acetyl cysteine therapy on cisplatin-induced testicular toxicity
- The Use of Generative AI for Scientific Literature Searches for Systematic Reviews: ChatGPT and Microsoft Bing AI Performance Evaluation
- Sildenafil as a Candidate Drug for Alzheimer’s Disease: Real-World Patient Data Observation and Mechanistic Observations from Patient-Induced Pluripotent Stem Cell-Derived Neurons
- Ozone-modified properties of pumpkin seed oil as anti-H. pylori, anticancer, anti-diabetic and anti-obesity agent
- Effectiveness of Low-Intensity Extracorporeal Shock Wave Therapy in Erectile Dysfunction: An Analysis of Sexual Function and Penile Hardness at Erection: An Umbrella Review
- Molecular Docking of Silymarin as a Ligand for Aromatase Enzyme and HIV-1 Reverse Transcriptase Using Molegro Virtual Docker
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.