Pathogenic diversity of Klebsiella pneumoniae strains with different serotypes and sequence types from human liver abscess
Abstract
BACKGROUND: Klebsiella pneumoniae (K. pneumoniae) strains exhibit diverse virulence phenotypes influenced by serotype (K-type) and sequence type (ST). However, the interplay between bacterial molecular/biological characteristics and specific pathogenicity remains poorly understood.
METHODS: We characterized 11 clinical K. pneumoniae isolates from liver abscess patients with varying serotypes (K1, K2, K5, K20, K57, K80), STs (including novel variants), and virulent factors. Phenotypic assays included the determination of growth curves, assessment of biofilm formation, and observation via electron microscopy. The Vitek 2 automated system was used to evaluate the minimum inhibitory concentration (MIC) of 11 clinical Klebsiella pneumoniae strains against common antibiotics. Mouse infection models were used to assess survival rates, analyze organ pathology, and detect hematological changes; these experimental data were complemented by clinical patient data.
RESULTS: Eleven clinically isolated Klebsiella pneumoniae strains displayed serotype/genotype-associated virulence and short bacilli morphology under TEM, with seven being hypermucoviscosity-positive. Growth and biofilm phenotypes varied: K2-ST65 (S2-029) had superior proliferation, K2-ST86 (S2-048) the weakest growth; K1-ST23 (S1-001) formed the strongest biofilm, while K57-ST218 (S57-077) the weakest. Phylogenetic analysis based on core SNPs derived from whole-genome sequencing of 11 K. pneumoniae strains revealed that…
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