Dual-channel SPR biosensor for enhanced glioma relapse diagnostics: Blood cell aggregation as a biomarker for tumor malignancy
Abstract
A biosensor based on the surface plasmon resonance (SPR) phenomenon has been developed for the express diagnosis of brain gliomas relapse by assessing blood cell aggregation indicators. The device features two optical channels, allowing for two studies to be performed simultaneously or enabling one channel to be used as a reference. This approach significantly increases biosensor sensitivity by reducing the impact of external factors. The optical excitation source is a p-polarized semiconductor laser with a 650 nm wavelength. The sensing elements were F1 optical glass plates with a refractive index of 1.61, with sputtered layers of chromium (5 nm) and gold (45…50 nm). As a result of the studies, a correlation was ascertained between the level of peripheral blood cell aggregation in patients and the degree of malignancy of gliomas. A statistically significant difference (p ≤ 0.05) was found between the groups of conditionally healthy individuals and those with grade II-IV gliomas. A decrease in the shift of the SPR curve during blood testing indicates an increase in cell aggregation levels and a decrease in their electric charge on membranes. This trend gradually intensifies with the increasing degree of glioma malig-nancy, reaching minimal values in patients with grade IV gliomas, indicating changes in the physicochemical properties of cell membranes and a reduction in their electric charge.
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