Ratiometric fluorescence probe integrated with smartphone for visually detecting lipopolysaccharide
Abstract
A portable instrument-free detection method for lipopolysaccharide (LPS) analysis was developed based on dual-emission ratiometric fluorescence sensing system. Herein, red-emitting Au nanoclusters (Au NCs) were as reference probe, while blue-emitting fluorescent silica quantum dots (Si QDs) were as response probe. Additionally, the aptamer of LPS was covalently grafted to the surface of Si QDs in order to specific recognize the LPS. According to the changes of fluorescence intensityratio (FL ratio, I461 nm/I643 nm) with the concentrations of LPS, the linear equation was fitted with the range of 50-3000 ng/mL, and the limit of detection (LOD) was 29.3 ng/mL. As a practical application, this method was employed to analyze LPS in normal saline with the recovery rate of 97.7-103.8 %. The color picker platform in the smartphone was used to transform the detection picture to the process of Red, Green and Blue (RGB) for visual detection of LPS. The low-cost and easy-carry method reported here presents broad merits for the visually quantitative detection of LPS.
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