Metabolomics-guided molecularly imprinted electrochemical sensor for noninvasive embryo quality assessment
Abstract
Accurate assessment of embryo quality remains a major challenge in the context of in vitro fertilization (IVF), since it significantly affects implantation success and clinical outcomes. Although metabolomics profiling of embryo culture medium (CM) holds promise for identifying biomarkers for embryo viability, no widely accepted indicators have yet been established. In this study, methylguanidine, L-valine, and oleic acid were identified as potential metabolic markers associated with embryo quality through semi-targeted metabolomics analysis of day 3 (D3) embryo CM. To enable sensitive and selective detection, a three-functional-module electrochemical sensing platform (TMEP) based on molecular imprinting was developed. An in situ grown RGO/Ni3(HITP)2 conductive metal-organic framework (MOF) composite was employed as the electrode material, which enhanced the conductivity and electrochemical performance of the sensing interface. The TMEP demonstrated high sensitivity, achieving detection limits of 2.3, 1.8 and 3.2 pM for methylguanidine, L-valine, and oleic acid, respectively. In addition, satisfactory recoveries (97.9%-104.8%, 95.4%-105.2%, and 97.2%-104.0%, respectively) and low relative standard deviations (RSDs <3.88%) confirmed its excellent reproducibility. Notably, the platform was successfully validated using clinical embryo CM samples, demonstrating its practical applicability for non-invasive embryo quality evaluation in IVF.
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.