3D bioprinting biomimetic small intestinal microtissue-based molecularly imprinted electrochemical sensor for sensitive detection of ovalbumin
Abstract
This study presents a novel sensing strategy for ovalbumin (OVA) by integrating a controllable molecularly imprinted polymer (MIP) fabrication technique with three-dimensional (3D) bioprinting technology. A MIP with high specificity and affinity was designed and synthesized for OVA using the reverse microemulsion epitope-directed surface imprinting and coating (ROSIC) method with silylating monomers. The synthesized MIP was then innovatively incorporated into gelatin methacryloyl (GelMA) and uesd as bioink to 3D bioprint a biomimetic small intestinal microtissue, which was subsequently assembled into a working electrode. Together with a homemade Ag/AgCl reference electrode and a platinum wire counter electrode, the resulting interface formed a three-electrode electrochemical sensing system for OVA detection. The constructed sensor exhibited a linear response to OVA concentration in the range of 10-400 pg/mL. The standard curve equation was Ret = 11.12 COVA (pg/mL) + 3261.9, with a correlation coefficient R2 of 0.998, and a detection limit of 5.54 pg/mL.
How strong is the evidence?
Evidence Hub reads this as a experimental / empirical study, done in animal, not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.