Preparation of highly sensitive electrochemical sensor for detection of nitrite in drinking water samples
Abstract
Nitrite is both an environmental contaminant and a food additive. Excessive intake of nitrites not only causes blood diseases, but also has the potential risk of causing cancer. Therefore, rapid detection of nitrite in water is necessary. In this work, we propose an electrochemical sensor for the sensing of nitrite. Glassy carbon electrodes modified with noble metal nanomaterials have been widely used in the preparation of sensors, but the surface properties of noble metals largely affect the sensing performance. This work proposes the biosynthesis of Au nanoparticles using the pollen extract of Lycoris radiata as a reducing agent. Flavonoids rich in pollen can be used as weak reducing agents for the reduction of chloroauric acid, and slowly synthesize uniformly dispersed Au nanoparticles. These Au nanoparticles do not agglomerate because they contain small biological molecules on the surface and can form a homogeneous sensing interface on the electrode surface. The electrochemical sensor assembled with biosynthesized Au nanoparticles provides linear detection of nitrite between 0.01 and 3.8 mM. The sensor also has excellent immunity to interference. In addition, the proposed sensor was also successfully used for the detection of nitrite in drinking water.
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