Nanozymes as signal amplifiers: a review of electrochemical sensing strategies for ultrasensitive detection of foodborne pathogens
Abstract
This study aims to systematically review recent advances in nanozyme-amplified electrochemical biosensors for ultrasensitive detection of foodborne pathogens. It highlights how nanozymes, as catalytic nanomaterials with enzyme-like activities, have revolutionized signal amplification strategies in pathogen detection, addressing the limitations of conventional methods such as lengthy analysis time and poor field applicability.
An extensive literature survey was conducted in Web of Science, Scopus, and PubMed (January 2010 to August 2025) using combinations of the keywords “nanozyme,” “enzyme-mimicking nanomaterial,” “electrochemical biosensor,” “foodborne pathogen” and specific pathogen names (e.g. Salmonella, Escherichia coli, Listeria monocytogenes, Campylobacter, Staphylococcus aureus, Vibrio). Original experimental studies were included when they employed nanozyme-based catalytic amplification in an electrochemical biosensor, targeted at least one bacterial foodborne pathogen, and reported analytical performance metrics. Based on this corpus, the review classifies nanozymes by catalytic function and material composition, critically analyzes the main electrochemical transduction mechanisms (voltammetry, amperometry, impedance spectroscopy), and synthesizes case studies to compare sensor performance, amplification strategies, and applicability in real food matrices.
Nanozyme-integrated electrochemical biosensors demonstrate remarkable sensitivity, achieving limits of…
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.