Organic electrolyte gated field effect transistor for detecting alpha synuclein longitudinally in Parkinsonism mouse model
Abstract
Parkinson's Disease (PD) is an age-progressive disorder caused by misfolding of alpha-synuclein (α-Syn) that can begin years before clinical symptoms appear, making early diagnosis crucial for timely intervention. In this study, a novel antibody-functionalized Organic Electrolyte-Gated Field-Effect-Transistor (Ab-OEGFET) biosensor was implemented to detect α-Syn levels in blood serum samples from an A53T Transgenic (TG) mouse line. PD-like pathology was examined in blood serum using Ab-OEGFET devices and in brain tissue samples using Western Blot and immunohistochemistry. Different forms (monomeric, phosphorylated, oligomeric) of α-Syn were identified in low volumes of blood serum samples collected from TG and Wild Type (WT) populations of mice at ages 2, 5 and 8 months, and the biosensor response was correlated to Blot and immunohistochemistry results. The Ab-OEGFETs performance in this study is a promising result towards a minimally invasive blood biomarker-based multianalyte testing strategy for early screening of PD and similar neurodegenerative disease pathologies.
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.