Detection of hepatitis B virus DNA in human serum based on highly sensitive electrochemical sensor
Abstract
The determination of specific DNA sequences in biological samples such as serum, organs, and body fluids is of great importance in biomedicine. Synthetic single-stranded DNA fragments of the Hepatitis B virus were immobilized as specific probes on the surface of gold electrodes using a self-assembled single-molecule membrane method. The electrode combined with the electroactive Hoechest 33258 constitutes the DNA electrochemical sensor. The film-forming conditions of self-assembled single-molecule membranes were explored during the immobilization of Hepatitis B specific DNA probes. The results showed that a probe concentration of 100 μg/mL and an immobilization time of 12 h was more favorable for the immobilization of the probe. We also explored the DNA immobilization and hybridization mechanism. We used a DNA electrochemical sensor for the qualitative and quantitative detection of standard concentrations of complementary DNA in solution. The results showed that the linear detection range could reach 0.05~1 μg/mL, and the limit of detection could reach 10 ng/mL when using the electrochemical sensor to detect standard DNA samples. On this basis, we have also successfully used DNA sensors for Hepatitis B virus detection serum. This allowed the fabrication of a DNA electrochemical sensor for the quantitative and qualitative detection of standard concentrations of complementary single strands and was performed on clinically obtained serum samples of Hepatitis B virus. We…
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.