CRISPR-Based Electrochemical Sensor Permits Sensitive and Specific Viral Detection in Low-Resource Settings
Abstract
Globally, over 300,000 women die from cervical cancer each year; 90% of these deaths occur in lowand middle-income countries (LMICs). Virtually all cases of cervical cancer are caused by persistent infection with high-risk types of human papillomavirus (HPV). In May 2018, Dr. Tedros Adhanom Ghebreyesus, Director-General of the World Health Organization, announced a global call to eliminate cervical cancer and underscored the need to ensure that all girls are vaccinated against HPV and that every woman over 30 is screened and treated for precancerous lesions. Standard methods to screen for high-risk HPV require costly equipment, trained personnel, and advanced infrastructureresources that are not available in many LMICs. Similar challenges exist for detection of many viral illnesses. In this issue of ACS Central Science, Zamani and colleagues describe a novel, low-cost electrochemical sensor for viral detection that can potentially be manufactured and used in low-resource settings. They demonstrate sensitive and specific detection of HPV 16 and 18, the two most common high-risk HPV types. Notably, the authors demonstrate detection of HPV in patient samples. They also show the platform can be readily adapted to detect other viruses, including detection of a plasmid containing the HIV genetic sequence. As shown in Figure 1, the electrochemical sensor is used to detect viral genetic material in a three-step process. Traditional methods are first used to extract viral genetic…
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