Novel selective and sensitive electrochemical sensor for detection of ovarian cancer biomarker, Cathepsin L.
Abstract
Late detection of ovarian cancer is critical as it results in decreased life expectancy of patients. Screening methodology with a rapid turnover is required to ensure better patient outcomes. Till date, no point of care diagnostics exists capable of monitoring epithelial ovarian cancer. Cathepsin L is a classic early-stage biomarker demarcating the proliferation and transition of ovarian cancer. A novel label-free electrochemical diagnostic is proposed for detection of Cathepsin L in serum. Intrinsic fluorescence spectroscopy was used to confirm the interaction between Cathepsin L and probe, Cystatin C. Cystatin C was immobilized on screen-printed carbon electrode using 1-Ethyl-3-(3-dimethyl-aminopropyl carbodiimide and N-hydroxysuccinimide cross linker. Electrochemical fluctuations resulting from Cystatin-Cathepsin interaction was detected by cyclic voltammetry, differential voltammetry and electrochemical impedance spectroscopy within a timeframe of 20 min. The proposed diagnostic for Cathepsin L exhibited analytical performance with a limit of detection upto 70 pg mL-1 via electrochemical impedance spectroscopy in the dynamic detection range of 2 ng mL-1-20 ng mL-1. The platform indicated reliable reproducibility, selectivity, and stability. Furthermore, validation with spiked sera samples (n = 5) showed correlation of R2 = 0.98. The results indicate sensitive yet selective point of care diagnosis of Cathepsin L in serum.
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