Electrochemical detection of creatinine at picomolar scale with an extended linear dynamic range in human body fluids for diagnosis of kidney dysfunction
Abstract
BACKGROUND
Creatinine levels in different body fluids can serve as an important biomarker for kidney functioning relevant to prostate cancer and chronic kidney disease (CKD). Creatinine levels vary in concentration in different body fluids, such as blood, urine, and saliva. Unlike previously reported sensors, the developed creatinine sensor can be employed for all levels of creatinine in samples of real patients.
RESULTS
In this study, an efficient voltammetric sensor for creatinine is developed by modifying a glassy carbon electrode (GCE) with poly (ethyleneimine) (PEI) capped silver nanoparticles at titanium dioxide (PEI-AgNPs)/TiO2, i.e., titanium dioxide (TiO2)/graphene oxide (GO) nanocomposites (Ag@GO/TiO2-GCE). The Ag@GO/TiO2 nanocomposite was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FT-IR) spectroscopy, and UV-Vis spectrophotometry. Various voltammetric techniques namely cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), chronoamperometry (CA), and differential pulse voltammetry (DPV) were employed. The Ag@GO/TiO2-GCE demonstrated good selectivity, stability, and a quick response time of 1.0 s for creatinine. An extended linear dynamic range (LDR) of creatinine from 0.01 pM (DPV) to 1.0 M (CV) based on different voltammetric…
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.