Mo2CT x MXene-based non-enzymatic electrochemical sensor for selective detection of hydrogen peroxide in colorectal cancer cells
Abstract
Accurate and real-time monitoring of hydrogen peroxide (H2O2) in biological environments is critical for understanding redox-regulated processes associated with cancer progression. In this study, we developed, for the first time, a novel non-enzymatic electrochemical sensor based on two-dimensional molybdenum carbide MXene (Mo2CT x ) for the selective detection of H2O2, with particular importance in human cancer cell applications. The synthesized Mo2CT x MXene was broadly characterized to confirm its layered morphology, surface terminations, and structural integrity. When coated onto a glassy carbon electrode (GCE), the MXene-modified glassy carbon electrode (MX-GCE) exhibited excellent electrocatalytic activity toward H2O2 reduction at a low operating potential of -0.45 V. The MX-GCE demonstrated a linear response range from 0.49 to 78.3 µM with a low detection limit of 1.02 µM and a rapid response time of ∼2 s. Interference analysis indicated that the MX-GCE exhibited high selectivity for H2O2 in the presence of common biological interferents. The practical applicability of the sensor was validated through real sample analysis by monitoring of extracellular H2O2 generated by cancer cells upon ascorbic acid induced stimulation. These results established that the Mo2CT x MXene modified electrode could be used as a stable and effective sensing platform for H2O2 detection in complex biological environments. This new sensor will be useful for oxidative stress monitoring and…
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