Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro
Abstract
Abstract A multiwalled carbon nanotube – chitosan (MWCNT‐Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5‐hydroxytryptamine (5‐HT). Electroanalytical response of the fabricated electrode towards the detection of 5‐HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT‐Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10−8–1.6×10−5 M and the low‐detection‐limit is 5×10−8 M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5‐HT in‐vitro directly from artificial urine samples and from diluted serum samples.
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