A Facile Ratiometric Electrochemical Sensor for In Vivo/On-line Repetitive Measurements of Cerebral Ascorbic Acid in Brain Microdiaysate
Abstract
The in vivo monitoring of ascorbic acid (AA) following physiological and pathological events is of great importance because AA plays a critical role in brain functions. The conventional electrochemical sensors (ECSs) usually suffered from poor se-lectivity and sluggish electron transfer kinetics for cerebral AA oxidation. To exploitation of ECSs adapt to the electrochem-ical detection (ECD)-microdialysis system, here we reported a facile ratiometric electrochemical sensor (RECS) for in vi-vo/on-line repetitive measurements of cerebral AA in brain microdiaysate. The sensor were constructed by careful electro-deposition of graphene oxide (GO) onto glassy carbon (GC) electrodes. Methylene blue (MB) was electrostatically adsorbed onto GO surface as a built-in reference to achieve ratiometric detection of AA. The subsequent proper electro-reduction treatment was able to readily facilitate the oxidation of AA at a relatively negative potential (-100 mV) and the oxidation of MB at separated potential (-428 mV). The in vitro experiments demonstrated that the RECS exhibited high sensitivity (de-tection limit: 10 nM), selectivity and stability towards AA determination, enabling in vivo/on-line repetitive measurement of cerebral AA in brain microdiaysate with high reliability. As a result, the designed RECS was successfully applied in the ECD-microdialysis system to in vivo/on-line repetitive monitoring the dynamic change of cerebral AA in the progress of the global cerebral ischemia…
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