Solutions to Overcome Competitive Displacement of Oligonucleotides from Nucleic Acid-Based Electrochemical Sensors in Biofluids
Abstract
Nucleic acid-based electrochemical sensors (NBEs) can support continuous and highly selective molecular monitoring in biological fluids, both in vitro and in vivo, via affinity-based interactions. Such interactions afford a sensing versatility that is not supported by strategies that depend on target-specific reactivity. Thus, NBEs have significantly expanded the scope of molecules that can be monitored continuously in biological systems. Although sufficiently robust for continuous, multihour sensing of small molecules, peptides, and proteins in biological fluids both in vitro and in vivo, NBEs decay over periods longer than 12 hours of continuous operation if unprotected. Previous work has sought to deeply understand the mechanisms underlaying NBE sensor decay in biofluids, as a framework to later develop solutions that eliminate such degradation pathways. Here I present a biofluid mimetic that can be leveraged to specifically study competitive displacement of oligonucleotides from NBEs, a critical degradation pathway, and varying solutions to circumvent this degradation pathway.
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.