Development of electrochemical sensors for sensing of Dopamine
Abstract
In this thesis the development of an electrochemical sensor for sensing of Dopamine on the basis of redox cycling was pursued. A 3-dimensional, plane-parallel electrode array was designed, consisting of bottom and top electrodes, where pores provide a spatial confinement and electrode interface to the measurement solution. In a micromechanical bottom-up process, a multi-layered formation of electrically conducting gold structures and insulating silicon nitride passivation was created by optical lithography, vapor deposition and plasma-enhanced chemical vapor deposition. Pore-channels of 5 μm diameter were opened by reactive ion etching. The produced sensors were evaluated by redox-cycling measurements on potassium hexacyanoferrate via cyclic voltammetry and amperometry. Stable sensors could be produced with an inter-electrode distance of 300 and 400 nm and were further evaluated by amperometric measurements in a fluidic setup, investigating the temporal and spatial resolution on concentration-changes of dopamine in solution. The electrode fouling process of dopamine on gold electrodes could be shown for a concentration of 1 mmol/l dopamine. Repetitive potential switches between oxidative and reductive potential could not initiate a reversal of the process. For an in-vitro evaluation of the produced sensors dopaminergic PC12 cells were cultivated on the sensor surface and the release of physiological dopamine by the cells was induced by potassium chloride and dopamine. The…
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.