Simple characterization of acetylcholinesterase by electrochemical sensor
Abstract
The electrochemical sensors enable to simplify the complicated biochemical methods to procedures which can be routinely used. Acetylcholinesterase is an enzyme which is connected with neuron function in brain and with information propagation in living bodies. The low activity of enzyme or damaged enzyme is connected with senile demency and Alzheimer disease. The simple measurement of apparent enzyme activity and apparent Michaelis-Menten constant has been developed. The method has been tested in vitro in different solutions. The method needs numerical calculations and it is generally nonlinear and numerically unstable. The conditions of numerical stability have been defined. The conditions of numerical stability have been involved into the experimental procedure and they are automatically tested during measurement procedure. The method can selectively measure the properties of Acetylcholinesterase and Butyrylcholinesterase. The relation of both enzymes activities can be measured. The method has been tested on full blood and plasma. The measurement needs 10 to 25 /spl mu/l of blood (one drop of blood) and full time of analysis takes 15 minutes. It enables potential use of this method by practical physicians and it creates the tool for deep study of relation between Acetylcholinesterase properties and neurodegenerative diseases.
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