Electrochemical Sensor Based on Functionalized Multiwalled Carbon Nanotubes, Domperidone Determination, DNA Binding and Molecular Docking
Abstract
An electrochemical sensor based on modification of carbon paste electrode by the functionlized MWCNT (f-MWCNT/CPE) was prepared for determination of Domperidone in formulation and in human serum. f-MWCNTs were prepared by a simple surface oxidization of the pristine MWCNTs using a famous, less expensive and less vigorous KMnO 4 . The f-MWCNTs were characterized using FTIR spectra, TEM, BET N 2 sorption isotherms and TGA. Point of zero charge of pristine and oxidized MWCNTs was also determined. The surface morphology and charge transfer resistance of the bare and f-MWCNT/CPEs were investigated using SEM and EIS. The detection limit (4.68 × 10 −11 M) indicates the sensitivity of the developed electrode for trace assay of Domperidone in biological fluids. The binding interactions of Domperidone with DNA and the mode of this binding have been investigated using cyclic voltammetry, UV-vis spectrophotometry and molecular docking studies. The estimated binding free energy (–20.47 KJmol −1 ) by Autodock was comparable to the experimental free energy (–25.82 KJmol −1 ) in the cyclic voltammetric analysis. The minor groove-binding mode of interaction was also supported by docking simulation studies. The higher binding constant value (8.77 × 10 4 M −1 ) might be sufficient to interfere with DNA replication and thus, Domperidone can be used as an anticancer therapy.
How strong is the evidence?
Evidence Hub reads this as a experimental / empirical study, done in computer models (in silico), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
Read the paper
Related studies
- Nano Biosensors: Properties, applications and electrochemical techniques
- Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics
- Toward a Harmonization for Using in situ Nutrient Sensors in the Marine Environment
- Electrochemical determination of rutin by using NiFe2O4 nanoparticles-loaded reduced graphene oxide
- Application of Polyoxometalate-based composites for sensor systems: A review
- Sensitive Electrochemical and Thermal Detection of Human Noroviruses Using Molecularly Imprinted Polymer Nanoparticles Generated against a Viral Target
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.