Impact of Low-Dose X-Ray Radiation Exposure on Lung Cancer Progression
Abstract
Low-dose ionizing radiation is known to alter the biological properties of tumor cells. Although clinical computed tomography delivers low-dose radiation, the long-term radiobiological impacts of repeated low-dose X-ray exposure on the malignant progression of lung adenocarcinoma remain poorly defined and require further preclinical clarification. In this preclinical study, we established long-term passaged A549 lung adenocarcinoma cell models subjected to single and fractionated low-dose X-ray irradiation. In vitro functional phenotypes and molecular alterations were further evaluated using murine subcutaneous xenograft and tail vein pulmonary colonization models to assess tumor growth and lung colonization capacity. Long-term low-dose irradiation significantly promoted lung cancer cell proliferation and migration in vitro and enhanced tumor growth and pulmonary colonization in mice. The results for cells cultured in vitro showed that low-dose radiation induced persistent DNA damage responses, modulated p53 expression, and upregulated the expression of epithelial-mesenchymal transition (EMT)- and glycolysis-related proteins, thereby remodeling the aggressive malignant phenotype of lung adenocarcinoma cells. Prolonged low-dose X-ray exposure drives progressive malignant phenotypic remodeling in A549 cells. Our work provides experimental radiobiological evidence of radiation-promoted tumor progression. Importantly, these preclinical findings are restricted to cell culture and…
Read the paper
Related studies
- The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules
- X-ray-Computed Tomography Contrast Agents
- Reactivity of Surface Species in Heterogeneous Catalysts Probed by In Situ X-ray Absorption Techniques
- Single-crystal X-ray diffraction studies on structural transformations of porous coordination polymers
- Investigation of solid/vapor interfaces using ambient pressure X-ray photoelectron spectroscopy
- Resonant X-Ray Scattering Studies of Charge Order in Cuprates
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.