Creatine kinase function in mitochondria isolated from gravid and non-gravid guinea-pig uteri
Abstract
Mitochondria from gravid and non-gravid guinea pig uteri were isolated and respiratory rates examined to determine the responses to ATP, ADP and creatine. It was found that mitochondria isolated from gravid uterus had (i) a markedly higher respiration rate in state 3; (ii) a greater activation of respiration by creatine in the presence of 0.1 mM ATP and (iii) an elevated specific activity of mitochondrial creatine kinase. It was shown by a competitive enzyme method, using pyruvate kinase to trap ADP, that despite the presence of creatine kinase in the mitochondria, there is no functional coupling between mitochondrial creatine kinase and oxidative phosphorylation as has been shown for striated muscle. It is suggested that the function of uterine Mi-CK is to favour high energy phosphate turnover in conditions of increased metabolic demand in gestating uterine smooth muscle.
Read the paper
Related studies
- Biportal endoscopic versus microscopic lumbar decompressive laminectomy in patients with spinal stenosis: A randomized controlled trial
- A whey protein-based multi-ingredient nutritional supplement stimulates gains in lean body mass and strength in healthy older men: A randomized controlled trial
- Supplement-based nutritional strategies to tackle frailty: A multifactorial, double-blind, randomized placebo-controlled trial
- A randomized, double-blind, placebo-controlled, proof-of-concept trial of creatine monohydrate as adjunctive treatment for bipolar depression
- Creatine supplementation (3 g/day) and bone health in older women: a 2-year, randomized, placebo-controlled trial
- A Multi-Ingredient Nutritional Supplement in Combination With Resistance Exercise and High-Intensity Interval Training Improves Cognitive Function and Increases N-3 Index in Healthy Older Men: A Randomized Controlled Trial
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.