The estimation of creatine and of diacetyl
Abstract
Voges & Proskauer [1898] observed that when strong alkali was added to broth cultures of certain species of bacteria there developed, after an interval of time, a pink colour.Subsequently the work of Harden [1906] and of Harden & Norris [1911] established that the chemical reaction responsible was one between diacetyl and creatine (or certain similar substances).Attempts to make the reaction quantitative [Walpole, 1911; Eggleton & Eggleton, 1928] were only partially successful.The reaction was further studied by Duliere [1929], Lang [1932] and Muller [1935], but it was the discovery by Barritt [1936], that o-naphthol greatly intensifies the colour, which made it possible for the first timne to use this reaction as a means of estimating either creatine or diacetyl.The present paper presents some additional facts about the reaction and methods of estimation based on it. EXPERIMENTAL Preliminary experiment
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