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Cluster-set rest vs. traditional during repeated sprint training: effects on creatine phosphokinase activity, sprint abilities and 100-m sprint performance

Megahed M

PeerJ, 2026

Abstract

Background: This study examined the effects of cluster-set rest and traditional rest interval configurations during repeated sprint training on creatine phosphokinase enzyme (CPK) activity, sprint abilities, and 100-m sprint performance.

Methods: The study was carried out using a nonrandomized experimental design involving twenty well-trained male sprinters. Participants were assigned to two equally sized groups: the Cluster Set Rest Group (CSRG) and the Traditional Rest Group (TRG). The eight-week training intervention included 24 sessions, with three weekly training days. All variables were assessed pre- and post-intervention. CPK activity was assessed through biochemical analysis, while sprint abilities were evaluated using the 0-40 m sprint and repeated sprint ability 6 × 30-m (RSAT 6 × 30) tests. The 100-m sprint time (100 m SP time) was measured in accordance with the regulations set forth by world athletics.

Results: Significant group × time interactions were identified using a 2 × 2 mixed-factorial Analysis of Variance (ANOVA) for CPK activity (p p = 0.003; mean sprint speed: p = 0.010; fatigue index: p = 0.009), and 100-m SP time (p = 0.031), with the CSRG exhibiting significantly greater improvements than the TRG. CPK activity increased from 214.4 ± 40.45 U/L to 358.2 ± 18.74 U/L in the CSRG compared with 224.9 ± 29.03 U/L to 302.6 ± 13.84 U/L in the TRG. Likewise, 100-m SP time improved from 12.08 ± 0.25 s to 11.80 ± 0.16 s in the CSRG, whereas the TRG improved…

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