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Sustainable Development of Four O’ Clock Flower-Based Natural Food Colourant with Green Nanoparticle-Intensified Stability and Biofunctionality

R RC

2026

Abstract

Abstract Use of artificial colouring agents in sweets and packed food has become a common scenario now a day, which pose serious consequences in consumers especially children. Various studies around the globe reported the serious health hazards of artificial food dyes which include organ damage of liver, kidney and lungs thereby affecting metabolic enzymes, serum creatine and urea levels and brain damage resulting in neurotoxicity and even behavioural deficits in children. Food products which use artificial dye like soft drinks, ice cream, pastries, biscuits, cake, cookies etc. are more or less a part of modern generation’s daily food habit. Hence, it is imperative to search for alternative safe and healthy food additives of natural origin. In this direction, the current review focusses to exploit the feasibility of Mirabilis jalapa (four o’clock) flower extract as food colouring agent along with its possibility as a therapeutic agent. Phytochemical screening, Antioxidant activity evaluates the medicinally active substance and Optimization of the pigment. Therapeutic activity of the natural pigment will be determined by in vitro studies in animal cell lines. Nanoparticles of M. jalapa flower extract solution will be used for slow and sustained cellular delivery of the extract to study detailed physiological and biochemical interactions. Cellular delivery of iron nanocluster proven spectrophotometric analysis in the UV-visible range, SEM and XRD. The current investigation…

How strong is the evidence?

Evidence Hub reads this as a preprint, done in cell cultures (in vitro), not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.

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